Forage Evaluation Support Laboratory (FESL)
FESL/PPARP Mission
- to provide forage nutritive value analysis for interdisciplinary IFAS researchers throughout the State of Florida
- to support IFAS-wide plant breeders and geneticists by helping determine the DNA content of plant samples through the Plant Ploidy Analysis Research Program (PPARP)
- to provide hands-on training and experience in laboratory procedures to post-doctoral associates and undergraduate and graduate students
Analyses Offered
- Acid Detergent Fiber (ADF)
- Crude Fiber (CF)
- Dry Matter/Organic Matter
- Total Nitrogen
- In Vitro Organic Matter Digestion (IVOMD)
- Neutral Detergent Fiber (NDF)
- Neutral Detergent Fiber (NDF) with Ankom digester
- Total Phosphorus
Sample Submission Checklist
Sample Preparation
All samples should be dried at 60°C and ground through a 1 mm screen. Any material left on the screen should be rerun, not discarded or put into the sample bag, as particle size will greatly affect results (especially IVOMD).
Sample Packaging
Place samples in 6 oz. Whirl-Pak bags only. Fill bag approximately one-half full (between 10 and 25 grams). Bags with either too little or too much sample will make it difficult to obtain a representative sample for analyses.
Sample Labeling
Use a Sharpie, Magic-Marker, or other permanent marker to write the sample number directly on the sample bag. Place numbers on the bottom one-half of the bag.
Sample Numbering
Number all samples in the experiment from 1 to N. For experiments with greater than 400 samples please contact Dr. Lynn E. Sollenberger or Richard P. Fethiere to decide on the ordering of samples by reps or the potential for use of NIR.
Forms
- Complete FESL Form 1
- Complete FESL Form 2
- Ploidy Analysis Sheet
Location
Mail or bring samples to:
FESL
2088 McCarty Hall
P.O. Box 110300
Gainesville, FL 32611-0300
Questions for the Lab?
E-mail: rpfethiere@ifas.ufl.edu
Laboratory Management
- Lynn E. Sollenberger
Faculty Supervisor - Richard P. Fethiere
Coordinator of Laboratory Programs and Services