Neutral Detergent Fiber (NDF)
The procedure consists of boiling a 1-g sample in a neutral detergent solution for 1 h. The residue following filtration is the cell wall constituents. Acetone is used as the final extraction and drying step. Results are expressed as NDF (t), meaning the percentage of NDF on a DM basis. Results are also expressed as percent NDF(af), i.e., ash-free, meaning the percentage of NDF after correcting for the amount of ash in the sample.
Suggested Text Statement: Neutral detergent fiber (NDF) was determined using the procedure of Golding et al. (1985).
Golding, E. J., M. F. Carter, and J. E. Moore, 1985. Modification of the neutral detergent fiber procedure for hay. J. Dairy Sci. 68:2732-2736.